Journal: bioRxiv

Article Title: Enterococcus faecalis V583 LuxS/AI-2 system is devoid of role in intra-species quorum-sensing but contributes to virulence in a Drosophila host model

doi: 10.1101/344176

Figure Lengend Snippet: (A) VE14089; (B) VE14089 ΔluxS . Strains were grown in 2xYT medium. During growth, monitored by measuring OD at 600 nm, culture samples were taken and AI-2 concentration in the supernatant was measured using the calibration curve from .

Article Snippet: We studied the activity of LuxS in the strain VE14089 ( Rigottier-Gois et al ., 2011 ), which is a V583 derivative cured of its plasmids, and referred to as WT hereafter, a genetically tractable strain compared to the original V583 ( Matos et al ., 2013 ).

Techniques: Concentration Assay

Journal: bioRxiv

Article Title: Enterococcus faecalis V583 LuxS/AI-2 system is devoid of role in intra-species quorum-sensing but contributes to virulence in a Drosophila host model

doi: 10.1101/344176

Figure Lengend Snippet: Genes found to be affected by the luxS mutation, independent of (S)-4,5-dihydroxy-2,3-pentanedione addition, when compared to the parental strain VE14089, are grouped according to JCVI ( http://cmr.jcvi.org ) cellular main role. Differentially expressed genes with | log2-ratios | > 5.0 are presented (up-regulated,in light grey and shown as positive; downregulated,in dark grey and shown as negative). The genes with more than one cellular main role were counted twice.

Article Snippet: We studied the activity of LuxS in the strain VE14089 ( Rigottier-Gois et al ., 2011 ), which is a V583 derivative cured of its plasmids, and referred to as WT hereafter, a genetically tractable strain compared to the original V583 ( Matos et al ., 2013 ).

Techniques: Mutagenesis

Journal: bioRxiv

Article Title: Enterococcus faecalis V583 LuxS/AI-2 system is devoid of role in intra-species quorum-sensing but contributes to virulence in a Drosophila host model

doi: 10.1101/344176

Figure Lengend Snippet: VE14089 is represented in grey bars with full outline and VE14089 ΔluxS in white bars with a dashed outline. (A) Biofilm formation on polystyrene microtiter plates. The quantification of biofilm formation was assayed as a function of crystal violet stain (measured at 595 nm) retained by the biofilm biomass grown for 24 h. Data are mean of hexuplicate trials, and error bars indicate standard deviations. (B) VE14089 and VE14089 ΔluxS adhesion to Caco-2 cells. The results are presented as per cent adhesion ± standard deviation. (C) Percentage (± standard deviation) survival of growing cells of E. faecalis VE14089 and VE14089 ΔluxS at 2, 4 and 6 h of a challenge with 7 mM H 2 O 2. One hundred per cent corresponds to the number of CFU before H 2 O 2 treatment. All data are means (± standard deviation) of four independent experiments. (D) Time course of intracellular survival of VE14089 and VE14089 ΔluxS strains within murine J774A.1 macrophages. Results correspond to the percent means ± standard deviations of intracellular Survival Index (SI) determined at 0, 4, 8 and 24 h post-killing of external bacteria, of five independent experiments. 0 h post-killing corresponds to 2 h post-infection, so time points mentioned in the figure correspond to 2, 6, 10 and 26 h post-infection.

Article Snippet: We studied the activity of LuxS in the strain VE14089 ( Rigottier-Gois et al ., 2011 ), which is a V583 derivative cured of its plasmids, and referred to as WT hereafter, a genetically tractable strain compared to the original V583 ( Matos et al ., 2013 ).

Techniques: Staining, Standard Deviation, Bacteria, Infection

Journal: bioRxiv

Article Title: Enterococcus faecalis V583 LuxS/AI-2 system is devoid of role in intra-species quorum-sensing but contributes to virulence in a Drosophila host model

doi: 10.1101/344176

Figure Lengend Snippet: 25 Oregon R (5-to 7-day-old) male adult flies, raised at 25°C, were infected, by septic injury onto the thorax with a thin needle, with VE14089 and VE14089 ΔluxS strains. Data is representative of three independent experiments (75 flies per strain). Curves assigned with an * are significantly different (p< 0.0001) from the respective wild-type-infected curve, as determined by log-rank analysis.

Article Snippet: We studied the activity of LuxS in the strain VE14089 ( Rigottier-Gois et al ., 2011 ), which is a V583 derivative cured of its plasmids, and referred to as WT hereafter, a genetically tractable strain compared to the original V583 ( Matos et al ., 2013 ).

Techniques: Infection